subculture

Cells from frozen stock cultures are seeded in culture medium at an appropriate density and subcultured at least once before they are used in the in vitro 3T3 NRU phototoxicity test.
Les déclarants doivent seulement participer aux coûts des informations qu'ils doivent soumettre pour satisfaire aux exigences en matière d'enregistrement.
Cells from frozen stock cultures are seeded in culture medium at an appropriate density and subcultured at least once before they are used in the in vitro 3T3 NRU phototoxicity test.
Des cellules provenant de cultures mères congelées sont mises en culture à une densité appropriée dans le milieu de culture, et sont repiquées au moins une fois avant d'être utilisées pour le test de phototoxicité in vitro 3T3 NRU.
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